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Fig. S3
The ppp1cba/ppp1cbb knockdown phenotype is independent of p53. (A–C) Lateral views of representative 48 hpf zebrafish embryos injected with (A) 4 ng p53 MO, (B) 0.75 ng ppp1cba MO, 0.75 ng of ppp1cbb MO (2MO) and 4 ng p53 MO (C) a partially rescued embryo injected with 100 pg ppp1cbb mRNA, 0.75 ng of ppp1cbb, 0.75 ppp1cba MO and 4 ng p53 MO. (D) In addition, control embryos were injected with 4 ng control MO; 2MO and 4 ng control or 2MO, 100 pg ppp1cbb mRNA and 4 ng of control MO (D) Quantification of the truncated body axis phenotype in morphant and mRNA injected embryos. Each injection was performed multiple times with 25 embryos used to calculate body axis length and reported as % of uninjected clutch mates. Error bars are standard error, a black * indicates a statistically significant difference from control, # indicates a statistically significant rescue and a NS indicates two data sets that are not significantly different. Statistical significance was calculated using a one-factor ANOVA with Tukey post hoc analysis and is defined as p < 0.05.