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Fig. 1

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ZDB-IMAGE-130930-5
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Figures for Lin et al., 2013
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Fig. 1

The phenotype of the s1pr2as10 mutant.

(A-H) Lateral views of wild-type (WT) (A, C, E and G) and s1pr2as10 mutants (B, D, F and H). Pericardial edema (B′), small eyes, and tail blisters (B′) were observed in 72-hpf s1pr2as10 mutant embryos (B). Two separate hearts (white arrows, D) were detected at 24 hpf, whereas a fused heart (white arrow, F) was observed at 48 hpf in s1pr2as10 mutant embryos. Paraffin sectioning with haematoxylin and eosin staining revealed the presence of two atria and one ventricle in s1pr2as10 mutant embryos at 72 hpf (H). (I-M) Ventral views of wild-type (WT) (I), s1pr2as10 (J), mil (K), and the intercross mutant progeny of s1pr2as10 and mil mutants (L and M) at 24 hpf are shown. The arrows indicate the positions of the hearts. (N) Cardiomyocytes were labeled via cmlc2 staining. Two representative s1pr2as10 mutant embryos are shown. Both contacting and separated cardiomyocytes were detected in the s1pr2as10 mutant embryos from the 22 ss to 28 hpf. (O) Percentages of WT and s1pr2as10 mutants containing contacting myocardia at different developmental stages. Scale bar = 100 μm. The error bars indicate the standard error.

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