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Fig. 1
Lipid accumulation and pro-inflammatory cytokine level in zebrafish larvae.
A and B, Five-day old wild (A) and fli1:EGFP (B) zebrafish larvae were fed a 4% cholesterol-enriched (HCD) or a normal (control) diet for 10 days, both supplemented with a 10 μg/g red fluorescent lipid. Images of the caudal vasculature in live larvae show vascular lipid accumulation visualized by lipid-associated BODIPY 576/589 fluorophore deposits in HCD fed larvae. The larger lipid deposits were found at the bifurcation site (arrows). Scale = 40 μm. C. Increased expressions of IL-1β and TNF-α by 3 h LPS treatment at 2-day old zebrafish larvae. Semi-quantitative PCR was performed for IL-1β and TNF-α in triplicate (n = 20 zebrafish larvae per group) D. Semi-quantitative PCR for IL-1β and TNF-α expressions from zebrafish larvae fed a HCD enriched with 4% cholesterol or normal (control) diet for 8 days to 20 days. The experiment was performed in triplicate (n = 15~20 zebrafish larvae per group) E. Realtime RT-PCR analysis for the expression levels of IL-1β, TNF-α and IL-6 from zebrafish larvae fed a HCD enriched with 4% cholesterol or normal (control) diet for 10 days. Results are representative for at least three independent assays (n = 15–20 zebrafish larvae per one experiment).