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Fig. 3 Inflammation precedes pigment cell migration to a wound. (A-E) Confocal images gathered at different time-points to reveal recruitment of innate immune cells to the implanted bead in a zebrafish larva. (A) By 15 minutes after implantation a small number of neutrophils (mpo:GFP, green and yellow cells) and occasional macrophages (L-plastin-positive, red) are already present in the wound vicinity. The bead is highlighted in grey with the centroid as a white dot. (B) The peak of neutrophil recruitment is at 3 hpi. (C) By 6 hpi, many innate immune cells are observed around the bead and this time is approximately the peak of macrophage recruitment. (D) Inflammation persists for some time and a large number of leukocytes can still be found close to the bead at 12 hpi. (E) Inflammation has partially resolved at 24 hpi, but a few leukocytes are still lingering around the bead. (F) Timeline highlighting the sequential recruitment of different cell lineages to the bead implant in zebrafish larvae. Full coloured lines represent the period during which cells are recruited to the bead. Dotted lines represent the period when cells are migrating away from the bead. The transition between the full and dotted line marks approximately the peak of recruitment for each cell lineage. Scale bar: 50 μm.