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Fig. 8 Syndapin I and Cobl localize to the base of forming cilia. (A–M) Localization of GFP (A), GFP-syndapin I (D) and GFP-Cobl_N (G), as well as of a combination of mCherry-syndapin I (J) and GFP-Cobl (K) in ZF4 cells in relation to cilia marked by anti-acetylated-tubulin antibodies (B,E,H,L). In merged images, co-localizations appear yellow in (C,F,I) and white in (M). Scale bars: 5μm. (N) RT-PCR analysis of ZF4 cell RNA for syndapin I and cobl. DNA plasmids were used as positive controls (+control) and ef1alpha served as a technical control. Both syndapin I and cobl (marked) were detected. (O) Affinity-purified anti-syndapin I antibodies specifically recognize zebrafish GFP-syndapin I overexpressed in HEK293 cells in immunoblotting analyses. (P–R) Acetylated tubulin (P) and endogenous syndapin I (Q) in ZF4 cells. The merged image (R) additionally includes phalloidin (purple) and DAPI (blue) labeling. Insets, magnifications of boxed areas. Scale bar: 20 μm.