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Fig. 11
hCMV:eGFP transgene is expressed in ovary. (A) Transgene expression occurs in previtellogenic and vitellogenic oocytes within the ovary. (1) Fluorescence is mainly observed in the primary oocytes; (2) gfp transcripts were detected in the ovary of sexually mature transgenic female adults using the whole-mount RNA in situ hybridization and a digoxigenin-labeled antisense riboprobe. The gfp transcripts were observed in the cytoplasm of previtellogenic (pvO) and vitellogenic (vO) oocytes. No signal was observed in pre-ovulating oocytes (poO). Scale bar represent 150 μm. (B) Maternal inheritance of the GFP protein in the hCMV:eGFP transgenic zebrafish line. (1) The fluorescence is observed in the vitellus of stripped fully mature oocytes before fertilization; (2) one cell stage; (3) 2 cells stage; (4) 4 cells stage; (5) 32 cells stage; (6) 256 cells stage; (7) high; (8) 50% epiboly; (9) 75% epiboly; (10) 24 dpf embryo; (11) zygote at 256 cells stage resulting from the cross of a transgenic male and a wild type female shows no fluorescence; (12) Brightfield image of (11).
Reprinted from Gene expression patterns : GEP, 13(3-4), Mella-Alvarado, V., Gautier, A., Le Gac, F., and Lareyre, J.J., Tissue and cell-specific transcriptional activity of the human cytomegalovirus immediate early gene promoter (UL123) in zebrafish, 91-103, Copyright (2013) with permission from Elsevier. Full text @ Gene Expr. Patterns