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Fig. S1 The reh mutant intersegmental vessels start degenerating at 48 hpf and become absent or atretic by 72 hpf. Time-lapse confocal imaging of Tg(kdrl:GFP) reh and wild-type vasculature between 40-80 hpf shows that reh intersegmental vessels begin to degenerate at 48 hpf and are either absent or atretic by 72 hpf, whereas wild-type intersegmental vessels remain patent during the same time period. Time interval is every 15 minutes. Representative images are shown every 8 hours. Top, dorsal longitudinal anastomotic vessel; bottom, dorsal aorta/cardinal vein.
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