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Fig. 1 The zebrafish α-tocopherol transfer protein.
A. Alignment of human and zebrafish TTP amino acid sequences is shown. Double dots indicate identical residues and single dots correspond to similar amino acids. Red text signifies α-tocopherol binding pocket. Align2 software (http://bioinfo.cgrb.oregonstate.edu/fast a2.html. Accessed 2012 Sep 17.) was used for sequence comparison. Sequences were obtained from NCBI. B. Anti-human TTP antibody cross-reacts with TTP from adult zebrafish liver homogenate. The 33 kD zebrafish protein (left lane) shown with a Ttp-/- mouse sample as a negative control (right lane) and a WT mouse sample with the 32 kD mouse homolog (left lane).