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Fig. 2
Differentiation of flk1-GFP-Labeled Endothelial Cells in Primary Culture Flk1-GFP expressing endothelial cells are scattered at day 1 and become flatten and elongated at days 2–4, undergoing tube formation. After 5–6 days of culture, GFP-positive cells start to die (blue circles). (A and B) Primary cells stained by (A) DIC and (B) Hoechst 33342 (5 μg/ml for 4.5–5 hr) on day 6. See also Figures S1 and S2.
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