ZFIN Image: Jao et al., 2012, Fig. 8

Image

Figure Caption

Fig. 8

Wild-type human GLE1, but not the Fin_Major mutant form, rescues the defects caused by Gle1 depletion. Embryos from gle1^hi4161a/+ intercrosses were injected with a mix of two non-overlapping gle1 translation-blocking morpholinos (gle1 MOs). Following MO injection, the embryos were injected with buffer alone (gle1 MOs + Buffer) or with <140 pg of in vitro transcribed human GLE1 mRNA (wild type, gle1 MOs + hGLE1_WT; or the Fin_Major mutant form, gle1 MOs + hGLE1_Fin_Major) at the one-cell stage. The extent of head cell death – categorized as severe, mild or wild-type-like (WT-like) – was scored at 32 hpf. Only injecting wild-type GLE1 mRNA, but not the Fin_Major mutant form, rescues the head cell death. At least three independent injections were performed for each condition. Values are mean ± s.e.m. n, number of embryos analyzed in each condition.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development