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Fig. 2

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ZDB-IMAGE-120209-5
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Figures for Tobin et al., 2012
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Fig. 2

Extremes of LTA4H Expression Drive Hypersusceptibility in Zebrafish (A) Median number of macrophages recruited to the hindbrain ventricle of wild-type and LTA4H-high siblings 4 hr after injection of 150-200 M. marinum into this space at 30 hpf. p = 0.01 (Student′s unpaired t test). Representative of two independent experiments. (B) TNF mRNA levels (mean ± SEM of three independent experiments) for control and LTA4H-high siblings 1 dpi with 150-200 M. marinum. p = 0.02 by Student′s unpaired t test. (C) Fluorescence images of representative wild-type, LTA4H-low and LTA4H-high larvae 3 dpi with 90-100 M. marinum. (D) Bacterial burden of all larvae from (C) by fluorescence pixel counts (FPC). **p < 0.01; ***p < 0.001. (one-way ANOVA with Tukey′s post hoc test; all other comparisons not significant). Representative of > 7 independent experiments measuring differences in bacterial burden of the three genotypes. (E) Mean (±SEM) number of bacteria per infected macrophage in 11 wild-type larvae, 8 LTA4H-low larvae and 13 LTA4H-high larvae, 40 hpi with 150-200 erp mutant M. marinum. ***p < 0.001 (one way ANOVA with Dunnett′s post hoc test). Representative of two independent experiments. (F) Fluorescence images showing discrete bacterial clumps indicative of macrophage residence in wild-type versus corded extracellular bacteria in their LTA4H-low and LTA4H-high siblings at 3 dpi with 150-200 M. marinum. (G) Percentage of animals in (F) with cording among wild-type, LTA4H-low and LTA4H-high siblings 4 dpi after infection with 90-100 M. marinum. *p < 0.05; ***p < 0.001 (Fisher′s exact test comparing LTA4H-low and LTA4H-high to wild-type). (H) Quantitation of neutral red positive cells 4 dpi after infection with approximately 100 M. marinum in sibling controls and LTA4H-high animals and (I) sibling controls and LTA4H-low animals. See also Figure S2 and Movie S1, Movie S2, and Movie S3.

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Reprinted from Cell, 148(3), Tobin, D.M., Roca, F.J., Oh, S.F., McFarland, R., Vickery, T.W., Ray, J.P., Ko, D.C., Zou, Y., Bang, N.D., Chau, T.T., Vary, J.C., Hawn, T.R., Dunstan, S.J., Farrar, J.J., Thwaites, G.E., King, M.C., Serhan, C.N., and Ramakrishnan, L., Host genotype-specific therapies can optimize the inflammatory response to mycobacterial infections, 434-446, Copyright (2012) with permission from Elsevier. Full text @ Cell