ZFIN Image: Li et al., 2012, Fig. S5

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Figure Caption

Fig. S5

Hepatocyte apoptosis is not elevated in alr morphants. A–F, TUNEL assay performed on 4 dpf embryo liver sections. White dashed lines outline the liver. White arrowheads indicate some of the positively stained cells, which are undergoing apoptosis. Very low levels of apoptosis are found in the developing livers of wild type embryos and alr morphants. G, DNase treated sample from 30 hpf embryos, as a positive control. H, brain section from 30 hpf embryos, treated by heat shock (39 degree, 1 hour) to induce apoptosis, as a positive control. L: liver; I: intestine. (Yabu et al., 2001) Yabu, T., Todoriki, S., Yamashita, M., 2001. Stress-induced apoptosis by heat shock, UV and γ-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining. Fisheries Science 67, 333–340.

Acknowledgments

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