ZFIN Image: Zygmunt et al., 2011, Fig. 3

Image

Figure Caption

Fig. 3

Sema-PlxnD1 Signaling Ensures the Proper Endothelial Abundance of sflt1

(A) Alternative flt1 splicing yields sflt1 and mflt1 isoforms with unique eleventh exons. Exons, colored boxes. Introns, black lines.

(B) sflt1 encodes a soluble 474 aa protein. mflt1 encodes a 1,273 aa transmembrane protein. Protein domains: Immunoglobulin (Ig, red numbered boxes), transmembrane (TM, gray box), tyrosine kinase (TK, pink box).

(C–H) WISH, embryo trunks (genotypes and ages indicated) hybridized with sflt1 (C and D, F and G) and mflt1 (E and H) riboprobes (blue).

(I) qPCR measurements. Relative mRNA abundance of sflt1, mflt1, and YFP (from Tg(flt1:YFP)^hu4624/+) in 28 hpf obd/+ (WT level = 1, dashed line). Error bars represent coefficient of variance ^*p < 0.05.

(J) ELISA-based quantification of FLT1 prepared from cell extracts of HUVECs treated with both VEGF and Sema3E and the control or PLXND1-targeting shRNAs. Error bars represent SEM. ^***p < 0.001.

(C–H) n = 10 embryos per riboprobe, stage and genotype. Pictures of representative examples of stainings observed (10/10 embryos in each category). Anterior, left; dorsal, up. Scale bars represent 50 μm. See Figure S3.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Cell, 21(2), Zygmunt, T., Gay, C.M., Blondelle, J., Singh, M.K., Flaherty, K.M., Means, P.C., Herwig, L., Krudewig, A., Belting, H.G., Affolter, M., Epstein, J.A., and Torres-Vazquez, J., Semaphorin-PlexinD1 Signaling Limits Angiogenic Potential via the VEGF Decoy Receptor sFlt1, 301-314, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell