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Fig. 5 Effects of temporal inhibition of Wnt signaling on swimbladder mesenchyme and smooth muscles.
(A, C) Expression of has2 in swimbladder mesenchyme at 72 hpf in wild type and hs:Dkk1-GFP fishes that were heat-shocked from 12 hpf. (B) Absence of has2 expression at 48 hpf in the putative swimbladder when hs:Dkk1-GFP larvae were heat-shocked from 12 hpf. (D) Expression of has2 in the size-reduced mesenchyme in hs:ΔTcf-GFP larvae heat-shocked from 36 hpf. (E–G) Expression of fgf10a at 72 hpf in mesenchyme of wild type (E), transgenic hs:Dkk1-GFP larvae heat-shocked from 12 hpf (F) and 60 hpf (G). (H) Expression of fgf10a in the size-reduced mesenchyme in hs:ΔTcf-GFP larvae heat-shocked from 36 hpf. (I–K) Expression of acta2 at 72 hpf in mesenchyme of wild type, hs:Dkk1-GFP larvae heat-shocked from 30 hpf and 36 hpf. Note absence of acta2 staining in (J) and the reduced size of swimbladder in (K). (L) Expression of acta2 in the size-reduced mesenchyme in hs:ΔTcf-GFP larvae heat-shocked from 48 hpf. Scale bar in (A) = 200 μm for all panels.