|
Fig. 10 Chemical inhibition of Wnt signaling and its effect on swimbladder development.
(A–F) Effect of Wnt inhibition on development of swimbladder epithelia. Et(krt4:EGFP)sq33-2 embryos were doped with 10 µM IWR-1 from various time points and then live-imaged at 72 hpf. Panel (A) shows normal swimbladder epithelium in a controls without IWE-1 treatment and panels (B–F) show the lack of swimbladder epithelium in embryos treated from 12 hpf and smaller swimbladders in embryos treated from 14 hfp (C), 16 hpf (D), 24 hpf (E) and 30 hpf (F). Dotted circle indicates the position of swimbladder. (G–J) Effect of Wnt signaling inhibition on development of different tissue layers of swimbladder. Control embryos were treated with 10 µM IWR-1 from 14 hpf and were assayed by WISH at 72 hpf. The swimbladder epithelium, mesenchyme, smooth muscle and mesothelium were marked by hb9 (G) , has2 (H), acta2 (I) and elvol1a (J) expression respectively. Inserted boxes present their expression in normally developed swimbladder in controls for comparison. (G) shows lateral view and (H–J) show ventral view. The red arrowheads indicate the swimbladder, whereas the red arrows indicate pancreas. Abbreviation: sb, swimbladder.