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Fig. S16 Time-lapse monitoring of membrane and glycan movement during mitosis. H2A-GFP transgenic zebrafish were microinjected into the blastomere cell at the one-cell stage with GalNAz and mRNA for memCherry. Embryos were allowed to develop to 10 hpf, at which point they were reacted with DIFO-647 (100 μM, 1 h) and imaged by confocal microscopy. Blue, H2A-GFP; green, memCherry; red, DIFO-647. A single z-plane fluorescence image is shown at each time indicated (min:s). Scale bar: 20 μm.
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