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Figure Caption
Fig. S1 Sox3dNLS disrupts nuclear localisation. (A) GFP-tagged WT Sox3 expressed from RNA injected at the 1- to 2-cell stage was almost exclusively located in the nucleus of zebrafish embryo cells, whereas the distribution of a Sox3dNLS-GFP fusion appeared identical to GFP alone, as seen in both the nucleus and cytoplasm. (Ba-d) Sox3dNLS-dsRED and dsRED alone showed similar distribution throughout the cell. When co-expressed, Sox3dNLS-dsRED caused a loss of nuclear localisation of WT Sox3-GFP, whereas dsRED alone did not (compare c with d). Scale bar: ∼10 μm.
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