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Fig. 5 Interaction between Sox3, Boz and Sqt. Animal pole views, dorsal to the right. (A) Both endogenous (a) and ectopic expression of gsc and chd induced by Sox3dNLS RNA injected at the 1- to 2-cell stage (b) was blocked by both boz (c,d) and sqt (e,f) MOs. (B) Compared with uninjected WT embryos (a), injection of WT sox3 RNA at the 1- to 2-cell stage and assayed at 4.5 hpf repressed expression of gsc and chd (b), whereas boz and/or sqt activated their expression (c,e,g). Co-injection of sox3 RNA with boz (d) or sqt (f) or both (h) RNAs resulted in intermediate levels of gsc and chd expression. (C) Model of Sox3 function in pathways that promote organizer formation. T-bars indicate inhibition and arrows indicate requirement for activation. Scale bars: ∼200 μm.