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Fig. 7 TS protein inhibited in vitro translation of zebrafish TS mRNA.
A rabbit reticulocyte lysate in vitro translation system was used to determine the interaction between mRNA and TS protein. Incubation of zebrafish TS mRNA with the reticulocyte lysate yielded a protein product with a molecular mass of approximately 35 kDa. A, Zebrafish TS mRNA was incubated in the rabbit reticulocyte lysate system without (lane 1) or with 100 ng of BSA (lane 2), luciferase (lane 3), human p53 protein (lane 4) or alpha-chymotrypsin (lane 5). B, Excess TS protein was included in the reaction mixture. Zebrafish TS mRNA was incubated in the translation system without (lane 1) or with 1 ng (lane 2), 10 ng (lane 3), 100 ng (lane 4) or 200 ng (lane 5) TS protein.