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Fig. 2 Time-dependent effect of 5-FU on TS expression.
Zebrafish embryos (n = 100) were untreated or treated with varying concentrations of 5-FU. A. Western immunoblot analyses were performed to evaluate TS levels in untreated zebrafish embryos or in embryos treated with 1.0 μM 5-FU for 8 h (lane 1), 16 h (lane 2), 24 h (lane 3) and 32 h (lane 4). β-actin was used as internal control. B. The quantification results of protein expression. The expression level of TS in untreated embryos at 8 h post-fertilization (hpf) was defined as 1.0. Each experiment was performed more than three times.
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