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Fig. 1 Expression profile of TS mRNA and protein in zebrafish embryos treated with 5-FU or ZD1694.
Zebrafish embryos were treated with various concentrations of 5-FU or ZD1694 for 24 h and the expression levels of TS mRNA and protein were evaluated. The TS mRNA and protein level after 5-FU (A, B) or ZD1694 (C, D) treatment is shown. A, Zebrafish embryos were untreated (lane 1) or exposed to 0.1 μM (lane 2), 0.2 μM (lane 3), 0.5 μM (lane 4) or 1.0 μM 5-FU (lane 5) for 24 h, and TS mRNA and protein expression was determined using Northern and Western blotting analysis respectively. C, TS mRNA and protein level in untreated embryos (lane 1) or embryos treated with 0.1 μM (lane 2), 0.2 μM (lane 3), 0.3 μM (lane 4) and 0.4 μM (lane 5) ZD1694, respectively. B and D, The quantification results of protein expression, which represent the protein expression level after 5-FU or ZD1694 treatment, respectively, is shown. The TS expression level in untreated embryos was defined as 1.0.