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Fig. 4 Pax2/stm-l interaction is involved in otolith formation. A: Binding ability of zebrafish Pax2 on the upstream region 1 of stm-l (for the sequence see Table 1) in presence of increasing concentrations of unlabeled competitor. B: Dorsal views of medaka embryos at stage 24. Misexpression of zebrafish pax2 leads to ectopic expression of stm-l gene close to the otic region (arrows). C: Lateral views of embryos at 4 days postfertilization (dpf). Misexpression of pax2 or PD-Vp16 (a transcription activation form of Pax2/5/8 protein family) constructs lead to ectopic formation of otoliths (arrows). Heat shocks were done before otoliths begin to form (stage 22). Numbers 1 and 2 indicate the endogenous otoliths.