ZFIN Image: Coffin et al., 2009, Fig. 1

Image

Figure Caption

Fig. 1 Neuromast hair cells have a minimal calcium requirement. Fish were incubated for 90 min in embryo medium with no added calcium or in embryo medium with moderate (900 μM) calcium (standard embryo medium). (A) SO2 neuromast from a 5 dpf fish incubated in medium with no added calcium (nominal Ca²⁺-free; left) or in normal embryo medium (right). Hair cell nuclei are labeled with YO-PRO-1 (blue) and hair cell cytoplasm with FM 1-43 (red). Arrows in the left panel point to condensed nuclei. Scale bar = 5 μm and applies to both panels. (B) Hair cells were counted in four neuromasts per fish and hair cell numbers were separately normalized to the number of hair cells in control neuromasts. The normalized average for all four neuromasts is shown here. N = 9–10 fish/treatment, bars are mean ± 1 SD. Hair cell number is significantly different between the two conditions (unpaired t-test, p ≤ 0.01).

Acknowledgments

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Reprinted from Hearing Research, 253(1-2), Coffin, A.B., Owens, K.N., Raible, D.W., and Rubel, E.W., Extracellular divalent cations modulate aminoglycoside-induced hair cell death in the zebrafish lateral line, 42-51, Copyright (2009) with permission from Elsevier. Full text @ Hear. Res.