Fig. 6

Fig. 6 Retinal image at the appropriate plane. Arrows indicate the edge of the image of the substage condenser aperture diaphragm (SCAD). (a–c) All three panels are micrographs of eyes, focused at the plane of the photoreceptor/pigmented epithelium interface. (a, b) These illustrate the same eye, from a 108 hpf fish, surgically isolated and positioned with the pupil looking into the condenser beam, away from the objective. The image of the SCAD is shown, pinched down in a and opened wide in b. (c) This is a dorsomedial view of a right eye in a live 85 hpf fish, lateral up and to the right, anterior up and to the left. The image of a grating inside the SCAD appears on the dorsal retina. The cone layer is evidenced in the granular texture, most visible in the bright stripes. (d, e) These are two views of the same field, at different planes of focus. (d) A freshly isolated lens (L) adheres to a fragment of the retina (R). The plane of focus is at the level of the lens center. (e) The plane of focus is at the back focal plane of the lens, showing a sharp image of a grating (finer than the one in c) inside a SCAD. (f) A dark-reared 128 hpf fish, showing that the image of the grating and the SCAD are in focus at the level of the cones, whose ellipsoids are visible (arrowheads). The inset shows a less magnified view of this same field. Scale bar in e applies to a, b, d, and e, and the one in f applies to c and f.