Fig. S7

Fig. S7 isl1^K88X mutant embryos are deficient for Isl protein. (A-D) Live images of 48-hpf isl1^K88X sibling and mutant embryos showing no apparent morphological phenotype. (E-J) Single z-scans of confocal images of 23-somite stage isl1^K88X Tg(cmlc2:eGFP) embryos after immunofluorescence staining with α-eGFP (E,H) and α-Isl (F,I) antibodies. (E-G) isl1^K88X sibling embryo; (H-J) isl1^K88X mutant embryo. Arrowheads in the overlay of α-eGFP (green) with α-Isl (red) indicate the eGFP^posIsl^pos cells in the cardiac disk and trigeminal sensory ganglia. In the control (E-G), Isl is expressed in cardiac cells located at lateral positions in the cardiac disk (white arrowhead) and in the trigeminal sensory ganglion (green arrowhead), described in Fig. 3A-C. Note that the nuclear Isl signal in the cardiac disk in H-J is absent. Scale bars: 80 μm.