|ZFIN ID: ZDB-IMAGE-080925-2|
Fig. 1 moks309 Mutants Have a Complex Retina Phenotype
(A and B) Whole-mount lateral views of live 4.5 dpf zebrafish larvae. In moks309 mutants (B), the eyes are smaller than in the wild-type (A), but external morphology is otherwise indistinguishable.
(C and D) Horizontal sections of 5 dpf retinas stained with DAPI (blue) and Zpr1 antibody (green). moks309 retinas (D) have an expanded GCL (dashed white lines), compared to wild-type (C), and no photoreceptors, as revealed by Zpr1 staining.
(E and F) Coronal sections of 3 dpf retinas stained by TUNEL assay. moks309 retinas (F) have increased apoptosis, particularly in the photoreceptor layer (asterisks), compared to wild-type (E).
(G and H) Horizontal sections of 48 hpf retinas expressing GFP driven by the atoh7 promoter (green) and stained with DAPI (blue) and HuC/D antibody (red). moks309 retinas (H) have an increased number of GFP-expressing RGCs compared to wild-type (G).
Scale bars, 500 μm (A and B) and 100 μm (C–H).
|Acknowledgments:||ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.|