ZFIN ID: ZDB-FIG-080925-1
Del Bene et al., 2008 - Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient. Cell   134(6):1055-1065 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 1 moks309 Mutants Have a Complex Retina Phenotype

(A and B) Whole-mount lateral views of live 4.5 dpf zebrafish larvae. In moks309 mutants (B), the eyes are smaller than in the wild-type (A), but external morphology is otherwise indistinguishable.

(C and D) Horizontal sections of 5 dpf retinas stained with DAPI (blue) and Zpr1 antibody (green). moks309 retinas (D) have an expanded GCL (dashed white lines), compared to wild-type (C), and no photoreceptors, as revealed by Zpr1 staining.

(E and F) Coronal sections of 3 dpf retinas stained by TUNEL assay. moks309 retinas (F) have increased apoptosis, particularly in the photoreceptor layer (asterisks), compared to wild-type (E).

(G and H) Horizontal sections of 48 hpf retinas expressing GFP driven by the atoh7 promoter (green) and stained with DAPI (blue) and HuC/D antibody (red). moks309 retinas (H) have an increased number of GFP-expressing RGCs compared to wild-type (G).

Scale bars, 500 μm (A and B) and 100 μm (C–H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP dctn1as309/s309; rw021Tg(TL) standard conditions Long-pec retinal ganglion cell layer retinal ganglion cell IFL
rw021Tg standard conditions Long-pec retinal ganglion cell layer retinal ganglion cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-elavl IHC dctn1as309/s309; rw021Tg(TL) standard conditions Long-pec retinal ganglion cell layer retinal ganglion cell
IHC rw021Tg(TL) standard conditions Long-pec retinal ganglion cell layer retinal ganglion cell
zpr-1 IHC TL standard conditions Day 5 retinal photoreceptor layer photoreceptor cell
IHC dctn1as309/s309(TL) standard conditions Day 5 Not Detected retinal photoreceptor layer photoreceptor cell
Phenotype Details
Fish Conditions Stage Phenotype
dctn1as309/s309(TL) standard conditions Day 4 eye decreased size, abnormal
Day 4 lens protruding, abnormal
Day 5 retinal ganglion cell layer retinal ganglion cell increased amount, abnormal
Day 5 retinal photoreceptor layer photoreceptor cell absent, abnormal
dctn1as309/s309; rw021Tg(TL) standard conditions Long-pec retinal ganglion cell layer retinal ganglion cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell