IMAGE

Fig. 2

ID
ZDB-IMAGE-080828-2
Genes
Antibodies
Source
Figures for Vervenne et al., 2008
Image
Figure Caption

Fig. 2 Knockdown of lpp disrupts convergence and extension movements. (A, B) Validation of the morpholinos: (A) quantitative RT-PCR using total cDNA from tailbud stage embryos as a template, shows an 80% reduction of lpp expression after injection of Lpp-MO/3e3i but not after injection of Lpp-MM/3e3i; (B) Lpp protein levels of prim-5 stage embryos are strongly reduced after injection of Lpp-MO/ATG but not after injection of Lpp-MM/ATG, as shown by quantification of the intensities of the protein bands after Western blot analysis (cumulative results of three experiments). Error bars represent standard errors of the mean. (C–L) Morpholinos (Lpp-MO/ATG and Lpp-MO/3e3i) or mismatch control morpholinos (Lpp-MM/ATG and Lpp-MM/3e3i) were injected at the one- to two-cell stage and morphology was assessed at different stages. (C–H) tailbud stage, lateral view, dorsal to the right; the anterior-most structure is indicated with an arrow. (I, J) 5-somite stage, lateral view, dorsal to the right. (I′, J′) 5-somite stage, dorsal view; drawings schematically show the notochord and somites of the embryos in the pictures. (K, L) prim-5 stage, lateral view, anterior to the left. Abbreviations: NI, non-injected; ML, mediolateral axis; AP, anterior–posterior axis. (M) Zebrafish embryos were injected with Lpp-MO/3e3i (5 ng) or synthetic mRNA (7.5 pg) encoding full-length Lpp, or were co-injected with Lpp-MO/3e3i (5 ng) and synthetic lpp mRNA (7.5 pg). At tailbud stage, the embryos were scored morphologically for the presence of C&E defects, as depicted in panels E and H. The phenotypes of the embryos of three independent experiments were scored and the percentages of normal embryos, embryos with C&E defects (“CE defects”) and embryos with defects other than C&E defects (“other defects”) are indicated. n, total number of embryos injected for each condition.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 320(1), Vervenne, H.B., Crombez, K.R., Lambaerts, K., Carvalho, L., Koeppen, M., Heisenberg, C.P., Van de Ven, W.J., and Petit, M.M., Lpp is involved in Wnt/PCP signaling and acts together with Scrib to mediate convergence and extension movements during zebrafish gastrulation, 267-277, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.