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Fig. 10

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ZDB-IMAGE-080729-9
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Figures for Craig et al., 2008
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Fig. 10 Relative concentrations of VDR in the intestine following treatment of adult male zebrafish with vehicle, 1α, 25(OH)2D3 or lithocholic acid (LCA). * p<0.05. Adult zebrafish were administered 25 ng of 1α, 25(OH)2D3 in 5 μl of propylene glycol, 25 ng of lithocholic acid in 5 μl of propylene glycol, or vehicle (5 μl of propylene glycol) parenterally. Twenty two hours later the fish were killed and tissues harvested as described. Equal amounts of protein (60 μg) were separated by SDS-PAGE using 10% acrylamide gels. Following electrophoresis, proteins were transferred to PVDF membranes and the VDR was detected by Western blotting methods as described.

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Reproduced from J Bone Miner Res 2008;23:1486-1496 with permission of the American Society for Bone and Mineral Research
Full text @ J. Bone Miner. Res.