IMAGE

Fig. S1

ID
ZDB-IMAGE-080410-20
Source
Figures for Kiener et al., 2008
Image
Figure Caption

Fig. S1 The asymmetric distribution of plasma membrane proteins is maintained in tjp3/zo-3 morphants. Epithelial cell polarization in the pronephric ducts. Immunofluorescence on cryosections: anti-Tjp3/Zo-3 (green) and anti-E-cadherin (red) (a-f); anti- PKCζ (green) and anti-Na+/K+-ATPase (red) (g-l). Control morpholino at 1 dpf (a, g) and 2 dpf (d, j); tjp3/zo-3 splice morpholino at 1 dpf (b, h) and 2 dpf (e, k) and tjp3/zo-3 translation blocking morpholino at 1 dpf (c, I) and 2 dpf (f, l). Note the strong reduction of Tjp3/Zo-3 protein from the apical side of the pronephric ducts in morphants (arrows), whereas E-cadherin or Na+/K+- ATPase staining on the basolateral membrane or PKCζ labeling on the apical membrane is not affected.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 316(1), Kiener, T.K., Selptsova-Friedrich, I., and Hunziker, W., Tjp3/zo-3 is critical for epidermal barrier function in zebrafish embryos, 36-49, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.