Zebrafish valves form through invagination. Larvae were stained with rhodamine-phalloidin and imaged using confocal microscopy at 76 (A) or 62 (E) hpf, or imaged using SPIM at 76 (B), 85 (C,D) or 63 (F) hpf. Although by confocal microscopy a superior endocardial cushion appears to be present (panel A), SPIM imaging shows that this structure is actually a valve leaflet (B-D). Arrow in panel A points to the presumed endocardial cushion and that in D to the valve leaflet. No intermediate stage of mesenchymal cells was observed by either confocal (E) or SPIM imaging (F). A, atrium; V, ventricle.
ZFIN wishes to thank the journal for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if
additional information is required.
Oops. Something went wrong. Please try again later.