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Fig. 4 Expression of ct1 in zebrafish embryos. Left side views for the whole mount, except where noted, with anterior up and dorsal to the right. (A) 4-cell stage, animal pole view. (B) 70%-epiboly stage. (C) 90%- epiboly stage. (D) 8-somite stage (13 h). ct1 mRNA was distributed ubiquitously in the embryonic tissues in (AD) while no signal was found in the YSL. (E)15-somite stage (16.5 h). (F) Ventral view of (E). (G) Dorsal view of (E). The expression was restricted in the somites, the optic vesicles and the CNS. (H) Prim-5 stage (24 h). (I) Dorsal view of (H). The expression of ct1 mRNA persisted in both the newly formed and the matrue somites. (J) Cross section of (H) through the line j, showing the expression in the somites. (K) Prim-15 stage (30 h), showing ct1 mRNA in the somites. (L) Long-pec stage (48 h), showing the expression of ct1 in the foregut. (M) Cross section of (L) through the line m, showing ct1 expression in the epithelial cells of the foregut. CNS, central nervous system; G, gut; OV, optic vesicle; SO, somite. Scale bar: 80 μm for (J) and (M), 250μm for others.