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Fig. 3 dscam protein is expressed early in development. (A) Immunoblot of anti-dscam polyclonal and monoclonal antibodies against Cos-7 cell lysates expressing dscam. Arrowhead indicates dscam at 220 kDa. (B) Secondary antibody alone, 32 cell stage. (C) α-dscam monoclonal, 32 cell stage. (D) Secondary antibody alone, 90% epiboly. (E) α-dscam monoclonal, 90% epiboly.
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Reprinted from Developmental Biology, 279(1), Yimlamai, D., Konnikova, L., Moss, L.G., and Jay, D.G., The zebrafish down syndrome cell adhesion molecule is involved in cell movement during embryogenesis, 44-57, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.