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Fig. 1 Generation of Dusp6 DNA construct and expression of d2EGFP in transgenic embryos. (A) Diagram showing the Dusp6 gene locus and the DNA construct used in generating transgenic zebrafish. (B, E, & H) dusp6 expression at oblong (B), dome (E), and shield stage. (C) d2EGFP mRNA expression at sphere stage. (D, F, G, I, & J) Tg(Dusp6:d2EGFP)pt6 embryos at dome (D), 30% epiboly (F & G), and shield (I & J) stage. (B-F & I) are lateral views and (H & J) are animal views. Red arrowheads mark dorsal region of the embryo.
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Full text @ BMC Dev. Biol.