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Fig. 1 Na,K-ATPase α2 is required for heart and gut LR patterning in zebrafish. (A-D) Live 2-day-old control (A), α2 morphant (B), Ncx4a morphant (C) and A23187-treated (D) embryos. (E-G) α2 morphants that exhibited normal (E), absent (F) or reversed (G) cardiac looping after 2 days of development. Ventral view, with anterior to the top. v, ventricle; a, atrium. (H-J) 2-day-old α2 morphants with normal (H), straight (I) or reversed (J) positions of gut, liver and pancreas. Dorsal view, with anterior to the top. li, liver; p, pancreas. (K) Bar chart detailing the heart and gut laterality phenotypes of uninjected embryos and those injected with CTL-MO, α2-MO, α2-SP-MO, Ncx4a-MO and Ncx4a-SP-MO. The effects of α2-SP-MO and Ncx4a-SP-MO on RNA processing are shown in the upper and lower panels, respectively, on the right.