ZFIN ID: ZDB-GENE-040426-2331
Gene Name: spastin
Gene Symbol: spast    Nomenclature History

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Previous Names: spg4 (1), zgc:85952

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(Including Attributions)
Gene Type: protein_coding_gene
Location: Chr: 1 Mapping Details/Browsers
Description: Predicted to have microtubule-severing ATPase activity and tubulin binding activity. Involved in several processes, including cytoplasmic microtubule organization; generation of neurons; and positive regulation of microtubule depolymerization. Predicted to colocalize with the microtubule cytoskeleton. Used to study hereditary spastic paraplegia. Human ortholog(s) of this gene implicated in hereditary spastic paraplegia 4. Orthologous to human SPAST (spastin).
Genome Resources: Alliance (1),  Gene:405851 (1),  Ensembl(GRCz11):ENSDARG00000024933 (2)
GENE EXPRESSION
All Expression Data: 3 figures from 3 publications
Directly Submitted Expression Data: 1 figure (1 image) from Thisse et al., 2004 [MGC:85952]
Wild-type Stages, Structures: Zygote:1-cell (0.0h-0.75h) to Hatching:Pec-fin (60.0h-72.0h)
 
MUTATIONS AND SEQUENCE TARGETING REAGENTS
Allele Type Localization Consequence Mutagen Suppliers
sa19591 Point Mutation Unknown Premature Stop ENU
  • European Zebrafish Resource Center (EZRC) (order this)
  • Zebrafish International Resource Center (ZIRC) (order this)
  • zf2203 Indel Unknown Unknown CRISPR
    zko988a Small Deletion Unknown Unknown CRISPR
    zko988b Indel Unknown Unknown CRISPR
    Sequence Targeting Reagents
    Targeting Reagent Created Alleles Publications
    CRISPR1-spast 1
    CRISPR2-spast 1
    MO1-spast N/A 2
    MO2-spast N/A 3
    MO3-spast N/A 3
    MO4-spast N/A 1
    DISEASE ASSOCIATED WITH spast HUMAN ORTHOLOG
    Disease Ontology Term Multi-Species Data OMIM Term OMIM Phenotype ID
    hereditary spastic paraplegia 4 Alliance Spastic paraplegia 4, autosomal dominant 182601
    DISEASE ASSOCIATED WITH spast VIA EXPERIMENTAL MODELS
    Human Disease Fish Conditions Citations
    hereditary spastic paraplegia WT + MO3-spast standard conditions Julien et al., 2016
    GENE ONTOLOGY
    Ontology GO Term
    Biological Process axon extension (more)
    Cellular Component COLOCALIZES_WITH   microtubule cytoskeleton (more)
    Molecular Function alpha-tubulin binding (more)
    GO Terms (all 47)
    PROTEIN FAMILIES, DOMAINS AND SITES
    Type InterPro ID Name
    Conserved_site IPR003960 ATPase, AAA-type, conserved site
    Domain IPR003593 AAA+ ATPase domain
    Domain IPR003959 ATPase, AAA-type, core
    Domain IPR007330 MIT
    Domain IPR015415 Vps4 oligomerisation, C-terminal
    Domain IPR041569 AAA ATPase, AAA+ lid domain
    Family IPR017179 Spastin
    Family IPR035106 Spastin, chordate
    Homologous_superfamily IPR027417 P-loop containing nucleoside triphosphate hydrolase
    TRANSCRIPTS
    Type Name Length (nt) Analysis
    mRNA spast-201 (1)    Ensembl 2413
    Browsers: UCSCNCBIEnsemblZFIN
    INTERACTIONS AND PATHWAYS
    ANTIBODIES
    Name Type Isotype Host Organism Assay Source Publications
    Ab1-spast polyclonal Rabbit WB 2
    Ab2-spast polyclonal Rabbit Millipore
    2
    PLASMIDS No data available
    CONSTRUCTS WITH SEQUENCES FROM spast
    Construct Regulatory Regions Coding Sequences Species Tg Lines Publications
    tg(5xuas:spast-ha) UAS HAspast Danio rerio 1 1
    tg2(5xuas:spast-ha) UAS HAspast Danio rerio 1 1
    MARKER RELATIONSHIPS
    spast Contained in: [BAC] CH73-233A22 (1)
    spast Encodes: [cDNA] MGC:85952 (1)
    SEQUENCE INFORMATION
    Type Accession # Length (nt/aa) Analysis
    RNA RefSeq:NM_212915 (1) 2338 nt
    Genomic GenBank:CU651563 (1) 99177 nt
    Select Tool
    Polypeptide UniProtKB:Q6NW58 (1) 570 aa
    Sequence Information (all 15)
    ORTHOLOGY for spast ( Chr: 1 )
    CITATIONS (33)