ZFIN Gene: prpf8

ZFIN ID: ZDB-GENE-030131-577

ZFIN ID: ZDB-GENE-030131-577

Gene Name:

pre-mRNA processing factor 8

Gene Symbol:

prpf8 Nomenclature History

Previous Names:

id:ibd1257, ik:tdsubc_2a9 (1), im:7141966 (1), tdsubc_2a9, wu:fb37c02, wu:fb73e06, xx:tdsubc_2a9, zgc:56504

Gene Type:

protein_coding_gene

Location:

Chr: 15 Mapping Details/Browsers

Description:

Predicted to have pre-mRNA intronic binding activity and snRNA binding activity. Involved in spliceosomal tri-snRNP complex assembly. Predicted to localize to U5 snRNP and catalytic step 2 spliceosome. Is expressed in brain. Human ortholog(s) of this gene implicated in retinitis pigmentosa and retinitis pigmentosa 13. Orthologous to human PRPF8 (pre-mRNA processing factor 8).

Genome Resources:

Alliance (1), Gene:393951 (1), Ensembl(GRCz11):ENSDARG00000026180 (2)

GENE EXPRESSION
All Expression Data:	4 figures from 3 publications
Directly Submitted Expression Data:	1 figure (1 image) from Kudoh et al., 2001 [ibd1257]
	1 figure (1 image) from Thisse et al., 2004 [IMAGE:7141966]
	1 figure (1 image) from Thisse et al., 2004 [IMAGE:7137198]
Wild-type Stages, Structures:	Zygote:1-cell (0.0h-0.75h) to Hatching:Pec-fin (60.0h-72.0h)
	brain unspecified whole organism
High Throughput Expression:	GEO (1) Expression Atlas (1)

MUTATIONS AND SEQUENCE TARGETING REAGENTS

Allele	Type	Localization	Consequence	Mutagen	Suppliers
gl1	Point Mutation	Exon 8	Premature Stop	ENU
la019288Tg	Transgenic Insertion	Unknown	Unknown	DNA	Zebrafish International Resource Center (ZIRC) (order this)
sa1289	Point Mutation	Unknown	Premature Stop	ENU
sa2823	Point Mutation	Unknown	Premature Stop	ENU

Sequence Targeting Reagents

Targeting Reagent	Created Alleles	Publications
MO1-prpf8	N/A	1
MO2-prpf8	N/A	1

PHENOTYPE

Data:	3 figures from Keightley et al., 2013
Observed in:	brain central nervous system head mRNA splicing, via spliceosome myeloid leukocyte myeloid leukocyte differentiation pericardium post-vent region regulation of apoptotic process spinal cord spliceosomal tri-snRNP complex assembly

DISEASE ASSOCIATED WITH prpf8 HUMAN ORTHOLOG

Disease Ontology Term	Multi-Species Data	OMIM Term	OMIM Phenotype ID
retinitis pigmentosa 13	Alliance	Retinitis pigmentosa 13	600059

DISEASE ASSOCIATED WITH prpf8 VIA EXPERIMENTAL MODELS No data available

GENE ONTOLOGY

Ontology	GO Term
Biological Process	spliceosomal tri-snRNP complex assembly (more)
Cellular Component	nucleus (more)
Molecular Function	isopeptidase activity (more)

GO Terms (all 16)

PROTEIN FAMILIES, DOMAINS AND SITES No links to external sites available

TRANSCRIPTS
Type	Name	Length (nt)	Analysis
mRNA	prpf8-201 (1)	7534	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT

Browsers: UCSC, NCBI, Ensembl, ZFIN

INTERACTIONS AND PATHWAYS

ANTIBODIES No data available

PLASMIDS No data available

CONSTRUCTS WITH SEQUENCES FROM prpf8 No data available

MARKER RELATIONSHIPS

prpf8 Contained in:	[BAC] CH211-209M4 (1)
prpf8 Contains:	[SNP] rs3728726 (1), rs3728727 (1), rs3728728 (1)
prpf8 Encodes:	[EST] fb37c02, fb73e06, ibd1257, IMAGE:7137198 (1), IMAGE:7141966 (1), tdsubc_2a9 [cDNA] MGC:162871 (1)
prpf8 Has Artifact:	[cDNA] MGC:56504

SEQUENCE INFORMATION

Type	Accession #	Length (nt/aa)	Analysis
RNA	RefSeq:NM_200976 (1)	7541 nt	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Genomic	GenBank:CR522882 (1)	129430 nt	Select Tool MegaBLAST Ensembl
Polypeptide	UniProtKB:A0A0R4J8E6 (1)	2342 aa	Select Tool ZFIN BLAST SIB BLAST Ensembl Protein Blast

Sequence Information (all 18)

ORTHOLOGY for prpf8 ( Chr: 15 )

CITATIONS (33)

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