FIGURE

Fig. 2

ID

ZDB-FIG-240404-29

Publication

Hossain et al., 2024 - Establishment of a graphene quantum dot (GQD) based steroid binding assay for the nuclear progesterone receptor (pgr)

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Fluorescence characteristics of GQD-GST-zPgrLBD and competition of binding of P4-BSA-FITC with GQD-GST-zPgrLBD by steroids and their analogues.

(A) The fluorescent scanning pattern of free GQD-GST-zPgrLBD is indicated in blue line. Fluorescent scanning pattern of the reaction mixture with (orange line) or without free P4 (green line). The difference of fluorescence at 520 nm caused by the addition of free P4 is indicated by the double-sided arrow. (B) The dose-dependent effects of steroids (progesterone (P4), 17β-estradiol (E2), testosterone (T), cortisol) and their analogues (17,20β-DHP and Org OD 02) were determined using the established assay. An assay was performed in triplicate for each compound. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem Biophys Rep