Fig. 3

TMEM47 suppresses IFNφ1/ISRE activation induced by MAVS or STING. (A–D) EPC cells were seeded into 24-well plates overnight and co-transfected with MAVS-, STING-, TBK1-, or IRF3-expressing plasmid and pcDNA3.1-TMEM47 (250 ng or 250/500 ng) or empty vector, plus IFNφ1pro-Luc (A and C) or ISRE-Luc (B and D) at the ratio of 1:1:1. pRL-TK was used as a control. At 24 h post-transfection, cells were lysed for luciferase activity detection. (E–H) Overexpression of TMEM47 suppresses the expression of ISGs induced by MAVS and STING. EPC cells seeded in six-well plates overnight were transfected with 1 µg MAVS-Myc/STING-Myc plus 1 µg of TMEM47-HA or empty vector. At 24 h post-transfection, total RNAs were extracted to examine the mRNA levels of cellular epc ifn (E), epc vig1 (F), epc irf7 (G), and epc rig-i (H). The relative transcriptional levels were normalized to the transcriptional level of the β-actin gene and were represented as fold induction relative to the transcriptional level in the control cells, which was set to 1. Data were expressed as mean ± SEM, n = 3. Asterisks indicate significant differences from control values (*P < 0.05). All experiments were repeated for at least three times with similar results.