ZFIN Figure: Finotto et al., 2023, Figure 6

Figure 6

GSCC‐specific morphometrics and dynamics of the tumor and its microenvironment in 3D over time

Schematic overview of features extracted and computed from the time‐lapse movies of the orthotopic zebrafish xenograft model.

Tumor volume at the start of 1 and 5 dpi time‐lapse movies (n = 10 (BT333), 11 (BT569), 9 (CME037), 6 (CME038), 3 (LBT001), 18 (LBT003), 4 (LBT070), 11 (LBT123) zebrafish embryos; P = 0.0005 (BT333), 1.2e‐10 (BT569), 0.0001 (CME037), 0.0147 (CME038), 0.9905 (LBT001), < 1.0e‐15 (LBT003), 0.0099 (LBT070), < 1.0e‐15 (LBT123)).

Mean number of round GAMs over time, during 1 dpi movies (left) and 5 dpi movies (right) (n = 10 (BT333), 12 and 11 (BT569; 1 and 5 dpi), 10 and 9 (CME037; 1 and 5 dpi), 6 (CME038), 8 and 4 (LBT001; 1 and 5 dpi), 22 and 18 (LBT003; 1 and 5 dpi), 12 and 9 (LBT070; 1 and 5 dpi), 15 and 11 (LBT123; 1 and 5 dpi) zebrafish embryos, see also Fig EV3A).

Number of round GAMs at the start of 1 and 5 dpi time‐lapse movies (n = 10 (BT333), 11 (BT569), 9 (CME037), 6 (CME038), 3 (LBT001), 18 (LBT003), 4 (LBT070), 11 (LBT123) zebrafish embryos; P = 1.0000 (BT333), 0.9996 (BT569), 0.6715 (CME037), 0.0804 (CME038), 1.0000 (LBT001), 1.0000 (LBT003), 0.5888 (LBT070), 7.3e‐5 (LBT123)).

Mean number of ramified GAMs over time, during 1 dpi movies (left) and 5 dpi movies (right) (n = 10 (BT333), 12 and 11 (BT569; 1 and 5 dpi), 10 and 9 (CME037; 1 and 5 dpi), 6 (CME038), 8 and 4 (LBT001; 1 and 5 dpi), 22 and 18 (LBT003; 1 and 5 dpi), 12 and 9 (LBT070; 1 and 5 dpi), 15 and 11 (LBT123; 1 and 5 dpi) zebrafish embryos, see also Fig EV3A).

Number of ramified GAMs at the start of 1 and 5 dpi time‐lapse movies (n = 10 (BT333), 11 (BT569), 9 (CME037), 6 (CME038), 3 (LBT001), 18 (LBT003), 4 (LBT070), 11 (LBT123) zebrafish embryos; P = 4.7e‐5 (BT333), 0.3089 (BT569), 4.1e‐5 (CME037), 0.0081 (CME038), 0.2899 (LBT001), 9.2e‐5 (LBT003), 0.9758 (LBT070), 9.4e‐5 (LBT123)).

Mean GAM distance to the tumor over time, during 1 dpi movies (left) and 5 dpi movies (right) (n = 388 and 494 (BT333; 1 and 5 dpi), 503 and 471 (BT569; 1 and 5 dpi), 234 and 455 (CME037; 1 and 5 dpi), 107 and 251 (CME038; 1 and 5 dpi), 226 and 169 (LBT001; 1 and 5 dpi), 945 and 934 (LBT003; 1 and 5 dpi), 453 and 338 (LBT070; 1 and 5 dpi), 774 and 543 (LBT123; 1 and 5 dpi) GAMs).

Boxplot of GAM distance to the tumor of all GAMs within 30 μm of the tumor, at the start of 1 and 5 dpi time‐lapse movies, ranked by increasing median distance at 1 dpi (n = 634 and 302 (LBT003; 1 and 5 dpi), 458 and 115 (LBT123; 1 and 5 dpi), 66 and 48 (CME038; 1 and 5 dpi), 267 and 264 (BT333; 1 and 5 dpi), 309 and 98 (BT569; 1 and 5 dpi), 257 and 99 (LBT070; 1 and 5 dpi), 134 and 142 (CME037; 1 and 5 dpi), 109 and 26 (LBT001; 1 and 5 dpi) GAMs; boxes stand for 50% of the data and minima/maxima are indicated by the line ends).

Data information: Data describe biological replicates, i.e. individual zebrafish embryos. The xenograft experiment was replicated once for CME038, twice for BT333, BT569, CME037, LBT001, three times for LBT070 and LBT123, and four times for LBT003. In (B, D and F), data are presented as mean ± SD. The P‐values were calculated by two‐way repeated measures ANOVA, followed by Šídák's multiple comparisons correction. ns ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Source data are available online for this figure.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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