FIGURE

Fig. 5

ID

ZDB-FIG-230904-5

Publication

Bi et al., 2023 - Polysarcosine-based lipid formulations for intracranial delivery of mRNA

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Fig. 5

GFP expression within 2.5 dpf zebrafish embryos at 24 hpi in the dorsal view. 1.5 nL lipoplexes (DOTAP/DOPE/DSPE-Rhodamine B/pSar-lipid or DSPE-PEG2k, 30 mM, N/P 10) containing GFP mRNA (100 ng/μL) were injected into the zebrafish brain. (A-C) GFP (green) expression in wildtype ABTL zebrafish embryos that were injected with (A1-A4) 2.5% C14-pSar2k, C14-pSar5k, C18-pSar2k and DSPE-PEG2k lipoplexes (magenta) and (B1-B4) 5.0% C14-pSar2k, C14-pSar5k, C18-pSar2k and DSPE-PEG2k lipoplexes in dorsal view (scale bar: 200 μm). (C) Quantification of GFP-expressing cells in the brain of zebrafish embryos after intracranial injection (n = 6). (D) GFP (green) expression within 2.5 dpf Tg(mpeg1: RFP) zebrafish embryos (macrophages in magenta) at (D1) 24 hpi, (D2) 48 hpi and (D3) 72 hpi in the dorsal view of brain (scale bar: 200 μm, 100 μm, 50 μm). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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