Fig 2

Age, but not ethanol and/or caffeine treatment, significantly affects eye diameter.

As in Fig 1, measurements of (A) inner and (B) outer eye diameter (in mm) were taken immediately after the 7-d exposure period to ethanol and/or caffeine. Measurements were pooled to assess the effect of age of sacrifice across these experimental conditions. Black bars = 1.5% ethanol; gray bars = control (0% ethanol). Caffeine exposures (0, 25, or 100 mg/L) are given along the x-axis. Data from individual fish were assessed in age bins to facilitate comparisons: 80–99 dpf (young adult); 100–119 dpf (adult). Despite age-dependent differences (asterisks), ethanol and/or caffeine exposure did not significantly impact measurements. The boxplots show the minimum, first quartile, median, third quartile, and maximum values for each measure after outliers were removed. Age when sacrificed (dpf) is indicated at the top of the bar graphs. For both measurements, there were 7–9 independent replicates per treatment condition (each ethanol/caffeine dose) for a total of 51 subjects. ANOVA tables and multiple comparison results for this data can be found in S1 Table, panels (E) Inner Eye Distance; (F1, F2) Outer Eye Distance.