FIGURE

Fig. 2.

ID

ZDB-FIG-230420-140

Publication

Yu et al., 2023 - Pax4-Ghrelin mediates the conversion of pancreatic ɛ-cells to β-cells after extreme β-cell loss in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2.

Overexpression of ghrelin or deletion of Pax4 potentiates β-cell regeneration. (A) Experimental scheme for DMSO or MTZ treatment of the wild-type, ghrelin^−/− or Tg(hsp70l:ghrl-HA-p2a-mCherry) larvae under the Tg(ins:CFP-NTR; ghrl:Dendra2) background. (B) Overexpression of ghrelin led to an increased number of neogenic CFP⁺ cells, including Dendra2⁺ CFP⁺ double-positive cells (arrowhead) compared with the wild-type MTZ-treated group, but not in the ghrelin^−/− mutant. (C) Numbers of CFP⁺ cells and proportions of Dendra2⁺ CFP⁺ double-positive cells per embryo at different stages (n=20). (D) Experimental scheme for DMSO or MTZ treatment of the wild-type, pax4^−/− or Tg(hsp70l:pax4-HA-p2a-mCherry) larvae under the Tg(ins:CFP-NTR) background. (E) The numbers of neogenic CFP⁺ cells were significantly increased in the pax4^−/− mutant, and overexpression of pax4 could rescue the increased β-cell regeneration in the pax4^−/− mutant. (F) The numbers of CFP⁺ cells per embryo at different stages (n=20). All statistical data are expressed as mean±s.e.m. P-values were calculated using an unpaired Student's t-test. Scale bars: 10 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development