Fig. 8.
- ID
- ZDB-FIG-230420-128
- Publication
- Sigloch et al., 2023 - A network of Notch-dependent and -independent her genes controls neural stem and progenitor cells in the zebrafish thalamic proliferation zone
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Overexpression of Her4 and Her6 reveals differential cross-regulation of her genes. (A-H′) WISH for her gene expression after heat shock-induced overexpression of Her4 or Her6. WT heat-shocked sibling controls (A-H), Tg(hsp:her4-FLAG) (A′-D′) and Tg(hsp:her6-FLAG) (E′-H′) transgenic embryos. Larvae were heat shocked at 70 hpf for 30 min and fixed at 72.5 hpf, except for G and G′ that were fixed at 71.5 hpf because her6 transcript levels decrease faster after heat shock than her4 transcript levels. her4 probe detects her4.1-4.5. her15 probe detects her15.1 and her15.2. Sagittal optical sections close to midline, anterior at left, dorsal up. pth, prethalamus; rh, rhombencephalon; tec, tectum opticum; tel, telencephalon; th, thalamus; ZLI, zona limitans intrathalamica. Scale bar: 100 µm. |