Fig. 2
- ID
- ZDB-FIG-230111-26
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- Bertozzi et al., 2021 - Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte regeneration in the zebrafish heart
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Fig. 2. Validation of IWR1 and of the hsp70l:Mmu.Axin1-YFP (hs:Axin1-YFP) transgenic line as tools to inhibit Wnt/β-catenin signaling in the adult zebrafish heart. (A) YFP immunofluorescence in cryoinjured hearts of hs:Axin1-YFP fish and wild-type siblings, harvested 6 h after a single heat shock (h.s.) at 7 dpi. n (hearts) = 6 (wild-type), 6 (hs:Axin1-YFP). In the overview, the magenta dashed squares represent the magnified portion of the myocardium. The yellow dotted lines represent the outline of the magnified MF20+ cardiomyocyte area, the cyan dotted lines represent the outline of the magnified MF20— area occupied by what we consider endothelial cells in the wound. Scale bars, 100 μm (overview), 10 μm (magnification). (B) axin2 RNAscope signals in MF20+ borderzone cardiomyocytes of hs:Axin1-YFP fish and wild-type siblings, harvested 6 h after single heat shock (h.s.) at 7 dpi. n (hearts) = 16 (wild-type), 16 (hs:Axin1-YFP). The yellow dotted lines represent the outline of the MF20+ cardiomyocyte area. Scale bars, 100 μm (overview), 10 μm (magnification). Error bars represent CI 95%. Unpaired Welch's unequal variances t-test. (C) Quantification of axin2 RNAscope signals in borderzone cardiomyocytes of cryoinjured hearts from fish treated with DMSO or IWR1 from 1 to 7 dpi. Error bars represent CI 95%. n = 8 (DMSO), 7 (IWR1). Unpaired Welch's unequal variances t-test. |
Reprinted from Developmental Biology, 481, Bertozzi, A., Wu, C.C., Hans, S., Brand, M., Weidinger, G., Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte regeneration in the zebrafish heart, 226-237, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.