Fig. 6

Figure 6. Template strand segregation in pectoral fins and kidneys (A) Zygotes were injected with 5.7 pmol F-ara-EdU as pulse; 10 mM BrdU was administered through incubation for 24 h before the end of the chase at 17–21 dpf. (B and C) Representative maximum intensity projections of Huygens-deconvolved F-ara-EdU/BrdU double-positive cell pairs (arrows) in pectoral fins (B) and kidneys (C). Partial BrdU staining, apparent as peripheral nuclear staining, was observed in some cell types, but this did not interfere with the identification of daughter cell pairs or F-ara-EdU quantification. (D) F-ara-EdU intensity ratios between daughter cells (n = 80 cell pairs). A binomial curve centered at 0.5 (magenta) was fitted to the data (R2 = 0.89). The blue curve represents the theoretical, normal (Gaussian) distribution for random segregation. Quantification was performed on raw images (see STAR Methods), whereas images shown in (B) and (C) were adjusted in brightness and contrast for better visualization.