FIGURE

Fig. 2

ID

ZDB-FIG-221209-21

Publication

Moss et al., 2021 - Autophagy coordinates chondrocyte development and early joint formation in zebrafish

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Fig. 2

atg13 mutants show reduced autophagy flux. (A) Representative single confocal z‐slices of epidermal cells taken from LysoTracker stained Tg(cmv:gfp‐map1lc3;atg13) wt and mutant larvae, at 4 dpf following treatment with DMSO or 100 nM Bafilomycin for 3 h. Scale bars = 10 µm. (B) Quantification of number of lysosomal (red) and GFP‐Lc3 (green) puncta per cell. Two‐way ANOVA performed for each; ****p < .0001. (C) Representative immunoblot of atg13 wt, heterozygous and mutant larvae at 5 dpf following treatment with DMSO or 100 nM Bafilomycin for 3 h. LE, long exposure; SE, short exposure. Molecular weight markers indicated on right hand side of immunoblots

Expression Data

Antibodies:	Ab3-map1lc3b Ab6-sqstm1 Ab16-GFP
Fish:	WT atg13^{fci500/fci500} atg13^fci500/+ zf155Tg atg13^{fci500/fci500}; zf155Tg
Condition:	chemical treatment by environment: bafilomycin A1
Anatomical Terms:	epidermal cell autophagosome whole organism
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atg13^{fci500/fci500} atg13^fci500/+ zf155Tg
Condition:	chemical treatment by environment: bafilomycin A1
Observed In:	epidermal cell autophagosome whole organism
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB J.