Figure 6.
- ID
- ZDB-FIG-221030-23
- Publication
- Wang et al., 2022 - Hoxa9/meis1-transgenic zebrafish develops acute myeloid leukaemia-like disease with rapid onset and high penetrance
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DHODH inhibitor leflunomide rescues defective haematopoiesis and improves the survival in Tg(drl:hoxa9;hsp70:meis1) fish. (a,b) Tg(drl:hoxa9;hsp70:meis1) embryos and control siblings were heat-shock induced at 24 hpf. Tg(drl:hoxa9;hsp70:meis1) embryos were treated with DMSO or Lef after heat shock. Embryos were assayed by WISH for the expression of cmyb, mpx and mfap4 at 3 dpf or 5 dpf. (c) Quantification of WISH results in (a,b) (n = 50–100 embryos per group). Data are represented as mean ± s.d. *p < .05, **p < .01, ***p < .001. (d) WT and Tg(drl:hoxa9;hsp70:meis1) were repeatedly heat-shock induced starting from 24 hpf, Tg(drl:hoxa9;hsp70:meis1) fish were treated with DMSO or Lef after the initial heat shock. Survival curves of WT and Tg(drl:hoxa9;hsp70:meis1) fish (n = 20–30 per group, Log-rank (Mantel-Cox) significance test). (e) WT and Tg(drl:hoxa9;hsp70:meis1) were repeatedly heat shock induced starting from 26 dpf, Tg(drl:hoxa9;hsp70:meis1) fish were treated with DMSO or Lef after the initial heat shock. Survival curves of WT and Tg(drl:hoxa9;hsp70:meis1) fish (n = 10 per group, Log-rank (Mantel-Cox) significance test). Lef, Leflunomide; hpf, hours post-fertilization; dpf, days post-fertilization. |