Figure 9

Activated polymorphonuclear leukocytes proteins orchestrate crucial inflammatory mechanisms 4h after λ-CGN injection in zebrafish. (A) The abundance of key proteins of cellular metabolism and kinetics, activity, and regulation is displayed. (A 1-12) Representation of the structural 3-D proteins in zebrafish (blue) plasma overlapping with their predicted Homo sapiens (red) homologous structures. Several inducers, mediators, and regulators of inflammation were reconstructed (E.g., Elongation factor 1-alpha, Galectin, or Fructose-bisphosphate aldolase, respectively). Note the presence and high abundance of Ezrin A in zebrafish. This protein is widely used to predict the strength of the inflammatory response in vertebrates. (B) The abundance of the main proteins related to the acute phase of inflammation triggered by the λ-CGN injection in zebrafish is presented. Note the marked increase in activity that was recorded in the apolipoprotein (classes: A, C, E, and M) and the parvalbumin isoforms (1, 2, 3, and 4). (B 1-8) Major circulatory proteins of the acute phase of inflammation are triggered by the main PMN cell family (Neutrophils, Eosinophils, Basophils, and Mast cells). The values in the graphs are expressed as relative abundance of the sample average (n = 4). The structural analysis of the proteins is described in the methodology. Abundance increases denoted by asterisks. Data are representative of two repeated trials in which all samples were run in triplicate (Student’s t test, p < 0.05). Error bars indicate SD.