FIGURE

Figure 1.

ID

ZDB-FIG-220323-5

Publication

Okada et al., 2022 - Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9

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Figure 1.

Establishing adequate quantities of CRISPR-Cas9 RNP complexes for crRNA evaluation. (A, B) Indel frequencies (in %) generated by different amounts of RNP complex with WT Cas9. 1 nl and 2 nl of 1.5 μM RNP complex (1.5 and 3 fmol, respectively) assembled using WT Cas9 with the indicated crRNAs were microinjected into 1-cell stage embryos, and indel frequencies were assessed with TIDE (A) and ICE (B). (C, D) Indel frequencies (in %) generated by different amounts of RNP complex with HiFi Cas9. TIDE (C) and ICE (D) were used for the assessment.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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